Mitochondrial DNA Isolation Kit from Abcam

Product Specs
ItemMitochondrial DNA Isolation Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65321
Quantity1 kit
SourceCell/Tissue
Sample TypeTissue, Adherent cells, Suspension cells
Time3h 0m
TargetMitochondrial DNA

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Mitochondrion-targeted carboxymethyl chitosan hybrid nanoparticles loaded with Coenzyme Q10 protect cardiac grafts against cold ischaemia‒reperfusion injury in heart transplantationJ Transl MedDecember 20, 2023Shun Yuan, Yanjia Che, Zhiwei Wang, Kai Xing, Xiaoping Xie, Yuanyang Chen(40778ES50) was acquired from Yisheng Biotechnology (Shanghai, China). A mitochondrial DNA isolation kit (ab65321) and protein carbonyl content assay kit (ab126287) were acquired from Abcam (Shanghai, China). A cardiac

(2) Myocardial Mitochondrial DNA Drives Macrophage Inflammatory Response through STING Signaling in Coxsackievirus B3-Induced Viral MyocarditisCellsOctober 31, 2023Andong Qin, Zhenke Wen, Sidong XiongIsolation Mitochondrial DNA was extracted using a commercial Mitochondrial DNA isolation kit (Cat#ab65321, Abcam, Cambridge, MA, USA). The experimental process was conducted in accordance with the experimental

(3) GSDMD promotes neutrophil extracellular traps via mtDNA-cGAS-STING pathway during lung ischemia/reperfusionCell Death DiscoveryOctober 4, 2023Chen Zhao, Fangte Liang, Mengling Ye, Siyi Wu, Yi Qin, Lu Zhao, Lu Zhang, Jing He, Liming Cen, Fei Linisolation and transfection mtDNA was purified from neutrophils via the mitochondrial DNA isolation kit (ab65321, Abcam, Cambridge, UK) following the manufacturer’s instructions. Neutrophils were transfected with

(4) Mitofilin Heterozygote Mice Display an Increase in Myocardial Injury and Inflammation after Ischemia/ReperfusionAntioxidants (Basel)April 13, 2023Yansheng Feng, Abdulhafiz Imam Aliagan, Nathalie Tombo, Jean C. BopassaCytosol The release of mtDNA in the cytosol was measured using the mtDNA isolation Kit from ABCAM (ab65321), which allows mtDNA isolation from cells and tissues in high yield and purity without contaminations

(5) Targeting PSAT1 to mitigate metastasis in tumors with p53-72Pro variantSignal transduction and targeted therapyFebruary 15, 2023Jiang J, Chen HN, Jin P, Zhou L, Peng L, Huang Z, Qin S, Li B, Ming H, Luo M, et al.purchased from Beyotime Biotechnology (C3601). Mitochondrial DNA Isolation Kit was purchased from Abcam (ab65321). Mitochondrial Respiratory Chain Complex Activity Assaying Kit was purchased from Solarbio (BC0510

(6) Disrupting metformin adaptation of liver cancer cells by targeting the TOMM34/ATP5B axisEMBO molecular medicineDecember 7, 2022Jin P, Jiang J, Zhou L, Huang Z, Qin S, Chen HN, Peng L, Zhang Z, Li B, Luo M, et al.fraction. Mitochondrial DNA (mtDNA) isolation mtDNA was isolated using Mitochondrial DNA Isolation Kit (Abcam, ab65321) according to the manufacturer's instructions. Briefly, 5 × 107 cells were resuspended in 1 ml cytosol

(7) Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signallingCell proliferationDecember 1, 2022Tu Q, Li Y, Zhu J, Guo L, Liu C, Liu L, Yuan Y, Zou Y, Chen F, Yao L, et al.isolated mitochondria were collected and used to isolate mtDNA by using the mtDNA isolation kit (Abcam, Ab65321) according to the manufacturer's instruction. Purified DNA concentration was determined via NanoDrop

(8) cGAS-STING activation contributes to podocyte injury in diabetic kidney diseaseiScienceOctober 21, 2022Nan Zang, Chen Cui, Xinghong Guo, Jia Song, Huiqing Hu, Mengmeng Yang, Mingyue Xu, Lingshu Wang, Xinguo Hou, Qin He, et al.Tunel Cell Apoptosis Detection Kit,Servicebio,Cat#G1507-100T Mitochondrial DNA Isolation Kit,Abcam,Cat#ab65321 PrimeScriptTM II Reverse Transcriptase,Takara,Cat#RR047 SYBR Green PCR kit,Takara,Cat#RR820 XF Cell Mito Stress Test Kit

(9) Genome Organization and Comparative Evolutionary Mitochondriomics of Brown Planthopper, Nilaparvata lugens Biotype 4 Using Next Generation Sequencing (NGS)Life (Basel)August 23, 2022Guru-Pirasanna-Pandi Govindharaj et al.sample and 10 individuals were taken for each sample) using the Abcam Mitochondrial DNA Isolation Kit (ab65321) according to the manufacturer’s protocol (Abcam, Cambridge, UK). Xcelris Labs Limited in Ahmedabad,

(10) Phenotypic but no genetic adaptation in zooplankton 24 years after an abrupt +10°C climate changeEvolution LettersAugust 1, 2022Antónia Juliana Pais‐Costa, Eva J. P. Lievens, Stella Redón, Marta I. Sánchez, Roula Jabbour‐Zahab, Pauline Joncour, Nguyen Van Hoa, Gilbert Van Stappen, Thomas Lenormandextraction (1984, 1997, and 2008). For each sample, mitochondrial DNA was extracted using an Abcam ab65321 Mitochondrial DNA isolation kit, following the manufacturer's instructions. NGS libraries were constructed

(11) Intravitreal injection of mitochondrial DNA induces cell damage and retinal dysfunction in ratsBiological researchJune 3, 2022Guo Y, Gan D, Hu F, Cheng Y, Yu J, Lei B, Shu Q, Gu R, Xu G.normal rat retinal cells as previously described [4]. According to the Mitochondrial DNA Isolation Kit (ab65321, Abcam, Cambridge, MA, USA), the isolated mtDNA was circular mtDNA about 15–20 kDa. The rat retinal

(12) Flubendazole induces mitochondrial dysfunction and DRP1-mediated mitophagy by targeting EVA1A in breast cancerCell death & diseaseApril 19, 2022Zhen Y, Yuan Z, Zhang J, Chen Y, Fu Y, Liu Y, Fu L, Zhang L, Zhou XL.China). Mitochondrial DNA quantification Mitochondrial DNA was isolated with a Mitochondria DNA Isolation kit (Abcam, ab65321) followed by PCR analysis using the mtDNA primers. The mtDNA primers were designed to detect MT-CO2

(13) mtDNA-STING Axis Mediates Microglial Polarization via IRF3/NF-κB Signaling After Ischemic StrokeFrontiers in ImmunologyApril 5, 2022Lingqi Kong et al.surgery (16, 26). The mtDNA (5mg/kg), extracted from liver tissue by Mitochondrial DNA Isolation Kit (ab65321, Abcam, USA), was intracerebroventricular injected (right lateral ventricle, bregma: 0.23mm posterior,

(14) Hyperoxygenation Ameliorates Stress-induced Neuronal and Behavioral DeficitsExperimental neurobiologyDecember 31, 2021Choi J, Kwon HJ, Seoh JY, Han PL.5’-CGAAGGTGGAAGAGTGGGAGTTG-3’. Mitochondrial DNA isolation was isolated using Mitochondrial DNA Isolation Kit (ab65321; Abcam, Cambridge, MA, USA), according to the manufacturer’s instructions. Tissue homogenate in cytosol

(15) PLK1 (polo like kinase 1)-dependent autophagy facilitates gefitinib-induced hepatotoxicity by degrading COX6A1 (cytochrome c oxidase subunit 6A1)AutophagyOctober 1, 2021Luo P, Yan H, Du J, Chen X, Shao J, Zhang Y, Xu Z, Jin Y, Lin N, Yang B, et al.cytometer. mtDNA quantitation mtDNA was isolated by using Mitochondrial DNA Isolation Kit (Abcam, ab65321) and genomic DNA was isolated by using EasyPure® Genomic DNA Kit (Transgene, EE101-02). To quantify

(16) Oxidized mitochondrial DNA sensing by STING signaling promotes the antitumor effect of an irradiated immunogenic cancer cell vaccineCellular & Molecular ImmunologySeptember 1, 2021Chunju Fang, Fei Mo, Li Liu, Jing Du, Min Luo, Ke Men, Feifei Na, Wei Wang, Hanshuo Yang, Xiawei Weias previously described.32 Mitochondrial DNA isolation was performed using the mtDNA Isolation Kit (#ab65321; Abcam), and gDNA isolation was performed using the QIAamp DNA Blood Mini Kit (#51106; QIAGEN) according

(17) Circulating mitochondrial DNA-triggered autophagy dysfunction via STING underlies sepsis-related acute lung injuryCell death & diseaseJuly 3, 2021Liu Q, Wu J, Zhang X, Li X, Wu X, Zhao Y, Ren J.from the livers of C57BL/6 mice subjected to ischemia and reperfusion using an mtDNA isolation kit (ab65321, Abcam, UK) following the manufacturer’s instructions. The DNA concentrations and purity were analyzed

(18) Morphological and complete mitogenomic characterisation of the acanthocephalan Polymorphus minutus infecting the duck Anas platyrhynchosFolia Parasitol (Praha)June 1, 2021Huda Sarwar, Wen-Ting Zhao, Caroline Jepkorir Kibet, Jiljí Sitko, Pin Nieand then stored in 95% ethanol at -80 °C, extracted by using Abcam Mitochondrial DNA extraction kit (ab65321; Abcam Trading, Shanghai), with suspended tissues in 1x Cytosol Extraction Buffer followed by treatment

(19) Enzymatic Noncovalent Synthesis for Mitochondrial Genetic Engineering of Cancer CellsCell reports. Physical scienceDecember 23, 2020He H, Lin X, Wu D, Wang J, Guo J, Green DR, Zhang H, Xu B.Mitochondrial DNA The mitochondrial DNA was extracted by a mitochondrial DNA isolation kit (Abcam, CAT#ab65321) following the manufacturer’s protocol. Western Blots of Mitochondrial Proteins Mitochondria were

(20) mtDNA-STING pathway promotes necroptosis-dependent enterocyte injury in intestinal ischemia reperfusionCell death & diseaseDecember 11, 2020Zhang X, Wu J, Liu Q, Li X, Li S, Chen J, Hong Z, Wu X, Zhao Y, Ren J.of 5 mg/kg mtDNA, which was extracted from liver tissues by using Mitochondrial DNA Isolation Kit (ab65321, Abcam) and mixed with EntransterTM-in vivo. The control group was intraperitoneally injected with EntransterTM-in

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Mitochondrial DNA Isolation Kit from Abcam

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Mitochondrial DNA Isolation Kit from Abcam

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